Altered Translation Elongation Contributes to Key Hallmarks of Aging in Killifish Brain
Domenico Di Fraia, Antonio Marino, Jae Ho Lee, Erika Kelmer Sacramento, Mario Baumgart, Sara Bagnoli, Till Balla, Felix Schalk, Stephan Kamrad, Rui Guan, Cinzia Caterino, Chiara Giannuzzi, Pedro Tomaz da Silva, Amit Kumar Sahu, Hanna Gut, Giacomo Siano, Max Tiessen, Eva Terzibasi-Tozzini , Eugenio F. Fornasiero, Julien Gagneur, Christoph Englert, Kiran R. Patil, Clara Correia-Melo, Danny D. Nedialkova, Judith Frydman, Alessandro Cellerino, and Alessandro Ori
Abstract
Aging is a major risk factor for neurodegeneration and is characterized by diverse cellular and molecular hallmarks. To understand their origin, we studied the effects of aging on the transcriptome, translatome, and proteome in the brain of short-lived killifish. We identified a cascade of events in which aberrant translation pausing led to altered abundance of proteins independently of transcriptional regulation. In particular, aging caused increased ribosome stalling and widespread depletion of proteins enriched in basic amino acids. These findings uncover a potential vulnerable point in the aging brain's biology, the biogenesis of basic DNA and RNA-binding proteins. This vulnerability may represent a unifying principle that connects various aging hallmarks, encompassing genome integrity, proteostasis and the biosynthesis of macromolecules.
Developed by Max Tiessen and Domenico Di Fraia
Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)Beutenbergstrasse 11 07745 Jena, Germany